International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

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Physico - Chemical Characterization of Alkaline Protease Produce by Bacillus Sp

Author(s) Jariwala Janvi N, Langalia Yash N, Parekh Sanjay N
Country India
Abstract In this study, Alkaline protease producing bacterial strains were isolated from various sample using skimmed milk agar. Among the isolates, Strain RE-1 shows highest protease activity which was identified as Bacillus sp. by partial 16S rRNA sequencing. The isolate showed maximum alkaline protease production (89.396 U/ml) at pH 8.0 and 30°C within 24 h of incubation. The enzyme exhibited thermal stability in the range of 40 to 70oC. Specific activity of partially purified enzyme is found to be 0.53 U/mg with 37.8% yield.
Keywords Alkaline protease, Enzyme Assays, 16S rDNA, Industrial effluent.
Field Biology > Bio + Chemistry
Published In Volume 6, Issue 3, May-June 2024
Published On 2024-06-06
DOI https://doi.org/10.36948/ijfmr.2024.v06i03.21347
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E-ISSN 2582-2160
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