Ancistrocline: A Naphthyl Isoquinoline Alkaloid from the Leaves of Ancistrocladus Heyneanus; Exhibits Anticancer Property Against PLC/PRF/5 Hepatoma Cells

Author(s)	Anil Karn, Anuradha Pandey Dubey, Sudhakar More, Madhuri Sharon
Country	India
Abstract	From the leaves of an Indian species of Ancistrocladus heyneanus, crude extract of mixed Naphthyl Isoquinoline alkaloids were tested against cytotoxicity and anti-cancer activity using MDCK 2 -cells and PLC/PRF/5 -cells. It showed >50% viability of MDCK 2 -cells, at all tried concentrations, showing it to be non-toxic to normal cells. Whereas, response to the viability of PLC/PRF/5 -cells at all concentrations of alkaloid exhibited a decrease in viability with increase in concentration, suggesting anti-cancer property of alkaloids. Since extract from leaves was a mixture of alkaloids, it was thought desirable to separate the alkaloids to see which alkaloid had anti-cancer property. We report anti-cancer property of separated Ancistrocline alkaloid. The alkaloids were separated using TLC, which showed 3-bands. Only middle band of the TLC bands, that has a molecular weight of 421 (as detected by LC/MS) and identified as Ancistrocline by NMR, has exhibited anti-cancer activity against PLC/PRF/5 -cells and was non-cytotoxic against MDCK 2 -cells
Keywords	Ancistrocladus heyneanus, Naphthyl Isoquinoline Alkaloids, Ancistrocline, MDCK 2 -cells, PLC/PRF/5 -cells, Anti-cancer
Field	Biology > Bio + Chemistry
Published In	Volume 6, Issue 3, May-June 2024
Published On	2024-06-08
DOI	https://doi.org/10.36948/ijfmr.2024.v06i03.22144

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