Micropropagation of an Arid Zone Leguminous Tree – Acacia Senegal (L.) Willd Via Callus

Author(s)	Parul Gupta
Country	India
Abstract	An efficient micropropagation system was developed for an economically important arid zone tree species-Acacia senegal (L.) Willd., commonly known as Gum Arabic. Callus induction and high frequency regeneration was obtained on Murashige and Skoog (MS) medium along with auxins, (NAA, 2,4-D, IAA), cytokinins (BAP, Kn) and additives like AA and AS. Rooting of the regenerated shoots was initiated on ½ MS-medium + NAA (2.5 mgl-1) + IBA (2.5 mgl-1) and low sucrose level (3%). However, further elongation and development of roots was achieved on ¼ MS + IBA (0.5 mgl-1) and still lower sucrose level (1%).For the hardening and acclimatization , the in vitro regenerated plantlets were transferred to a sterile vermiculite and sand mixture in a 3:1 ratio. Subsequently the hardened plantlets were transferred to a green house and finally to the fields.
Keywords	Micropropagation, Acacia senegal, Callus, differentiation
Field	Biology > Agriculture / Botany
Published In	Volume 3, Issue 6, November-December 2021
Published On	2021-11-08

About IJFMR Fees & Payment Current Issue Publication Archive	Submit Research Paper Track Submission Status Publication Guidelines Publication Ethics Peer Review & Plagiarism	Join as a Reviewer Editors & Reviewers Reviewer Referral Program Get Reviewer Membership Certi.	Website/Journal Policies Usage Policy Content Policies Privacy Policy

Contact Us		+91-9687-828-838	editor@ijfmr.com

International Journal For Multidisciplinary Research