International Journal For Multidisciplinary Research

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Antibacterial Activity Test of Basil Seed Extract (Ocimum sanctum L.) Against Growth Staphylococcus epidermidis

Author(s) Marselina Chindi Claudia Diana, Kartini Lidia, Su Djie To Rante, Desi Indriarini
Country Indonesia
Abstract Background: Staphylococcus epidermidis is a normal flora found in almost all parts of the human body that under certain circumstances can cause infection. It is the most common major nosocomial pathogen associated with medical devices and is currently one of the leading causes of increased morbidity and mortality in hospitals. Staphylococcus epidermidis infections are usually treated with antibiotics, which are generally resistant, so alternative natural ingredients are needed to inhibit their growth. One alternative that can be used is basil seeds (Ocimum sanctum L.).
Objective: To determine the antibacterial activity of basil seed extract (Ocimum sanctum L.) against the growth of Staphylococcus epidermidis.
Methods: This type of research uses a true experimental design with a post-test only control group design. In this study there were treatment groups given basil seed extract with concentrations of 20%, 40%, 60%, 80%, and 100%, positive control clindamycin and negative control sterile distilled water with Staphylococcus epidermidis as the test bacteria. The antibacterial testing method used disc diffusion. The results were analyzed using the Kruskal-Wallis statistical test.
Results: The results of the antibacterial potential test of basil seed extract against the growth of Staphylococcus epidermidis showed that basil seed extract with concentrations of 20%, 40%, 60%, 80% and 100% did not form an inhibition zone around the disc paper.
Conclussion: Basil seed extract (Ocimum sanctum L.) has no antibacterial activity against the growth of Staphylococcus epidermidis.
Keywords Staphylococcus epidermidis, basil seeds, antibacterial
Field Medical / Pharmacy
Published In Volume 7, Issue 1, January-February 2025
Published On 2025-02-16
DOI https://doi.org/10.36948/ijfmr.2025.v07i01.37040
Short DOI https://doi.org/g84728
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