Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

Sonali Priyadarsini; Pritismita Mahalik; Dharitri Priyadarsini; Rajat Kumar Nayak

doi:10.36948/ijfmr.2023.v05i04.4268

Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

Author(s)	Sonali Priyadarsini, Pritismita Mahalik, Dharitri Priyadarsini, Rajat Kumar Nayak
Country	India
Abstract	Street food is the one which is sold by vendors with a stall or maybe shop. Here using five different varieties of street food (Panipuri, Bhel, Momo, Dahibara, Chocolate), using different microbial techniques. Different microbes (Gram +ve and Gram -ve) have been observed, and using medicinal plants like (Neem, Tulsi, Parijat) antimicrobial testing has been done which shows the zone of inhibition and calculated result show the high amount of antimicrobial agent is Nyctanthes arbor-tristis (Parijat) with a zone of inhibition of 15 mm, 26 mm, 22 mm, 27 mm, 13 mm, etc. for 5 different samples respectively. Studies have come across a previously unidentified microbial variety through the use of molecular methods mainly 16S rRNA sequencing. The identification of novel taxa, a shortage of sequences in nucleotide databases, the existence of species with similar and/or identical 16S rRNA sequences, and nomenclature issues brought on by the assignment of multiple genomovars to a single species or complex are all challenges that must be overcome. Sequence data are analyzed using insilico method. To describe the connections between different bacterial strains, phylogenetic trees can be employed. This can detect strains that are very closely linked to one another and show which bacterial strains have been transmitted to whom.
Keywords	Street food, 16S rRNA, Sequencing, Nyctanthes arbor-tristis, insilico analysis, MSA, Phylogenetic tree.
Field	Biology > Genetics / Molecular
Published In	Volume 5, Issue 4, July-August 2023
Published On	2023-07-07
Cite This	Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis - Sonali Priyadarsini, Pritismita Mahalik, Dharitri Priyadarsini, Rajat Kumar Nayak - IJFMR Volume 5, Issue 4, July-August 2023. DOI 10.36948/ijfmr.2023.v05i04.4268
DOI	https://doi.org/10.36948/ijfmr.2023.v05i04.4268
Short DOI	https://doi.org/gsfxnk

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Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

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