International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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A review on Proteomics: Tools and Techniques in analysis of Plant Metabolites

Author(s) Ms. Sreeparna Bhattacharyya, Ms. Swati Das
Country India
Abstract Worldwide, crop yields are lost due to abiotic and biotic stresses. A wide range of research is conducted on the genome, transcriptome, proteome, and metabolome levels to reveal the mechanisms of plant reactions in response to their interactions with the environment. These studies will provide data to define the biochemical and physiological mechanisms behind plant resistance or susceptibility to affecting factors/stresses. The field of plant proteomics focuses on large-scale functional analysis of plant proteins. Researchers use proteomics-based mass spectrometric techniques to breed new varieties of crops that are tolerant to affecting stresses and have good agronomic properties. Molecular and cellular pathways are better understood through the large-scale analysis of proteins that provides a more comprehensive picture of how living systems function. Despite the high dynamic range and difficulties in assessing low abundance proteins, proteome analysis is an extremely challenging task. Various methods have been employed to study proteins, including gel-based techniques, protein microarrays, mass spectrometry-based approaches like MALDI and SELDI, liquid chromatography with high and ultra-high resolution, and fourier transform ion cyclotron resonance mass spectrometry. This review will give a brief overview of some of these techniques and their most recent developments.
Keywords Proteomics, techniques, abiotic and biotic stresses, plant metabolites
Field Biology > Genetics / Molecular
Published In Volume 7, Issue 3, May-June 2025
Published On 2025-05-16
DOI https://doi.org/10.36948/ijfmr.2025.v07i03.42950
Short DOI https://doi.org/g9kffn
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