International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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Synergistic Larvicidal Activity of Bacillus cereus and Bacillus thuringiensis Against Yellow Fever Mosquito

Author(s) Mark Adrian Fernandez Tacadena
Country Philippines
Abstract Dengue fever continues to pose a serious public health threat worldwide, necessitating effective and sustainable vector control strategies. This study investigated the larvicidal potential of Bacillus cereus and Bacillus thuringiensis, known biological control agents against Aedes aegypti larvae, the primary dengue vector. Given concerns over the environmental impact and resistance associated with chemical insecticides, this research focused on evaluating these bacterial strains individually and in combination, with a particular interest in identifying any synergistic effects. A completely randomized experimental design was employed, wherein mosquito larvae were exposed to the bacterial treatments at 12, 24, and 48-hour intervals. Larval mortality was recorded, and statistical analyses, including ANOVA and t-tests, were used to assess treatment efficacy and determine lethal concentrations (LC₅₀). Results showed that both B. cereus and B. thuringiensis significantly reduced larval survival individually; however, their combination did not produce a synergistic effect. Statistical analysis confirmed no significant interaction between treatment and exposure time, indicating that the combined application does not enhance larvicidal efficacy beyond the individual effects. These findings contribute valuable insights to the development of environmentally sustainable mosquito control programs.
Keywords Aedes aegypti, Bacillus cereus, Bacillus thuringiensis, microbial larvicides, mosquito vector control, synergistic effect
Field Biology > Zoology
Published In Volume 7, Issue 2, March-April 2025
Published On 2025-04-30
DOI https://doi.org/10.36948/ijfmr.2025.v07i02.43574
Short DOI https://doi.org/g9g76z
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