Development and Validation of Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Lamivudine and Zidovudine in bulk and formulation.

Author(s)	Dr. Sachin Bhusari, Shivani Shinde, Pravin Wakte
Country	India
Abstract	The present research work demonstrates an analytical method development for simultaneous estimation of Lamivudine and Zidovudine in combined dosage form using Q-absorbance ratio concept. While method development, two different wavelengths one representing iso-absorptive point (271 nm) and other representing the λmax of Lamivudine (269 nm) were used. Optimum response was obtained in solvent system that comprises methanol and water in ratio of 60:40 v/v. Proposed UV method was found to be linear over the concentration range of 1-20 µg/ml for Lamivudine and that of 1-16 µg/ml for Zidovudine.
Keywords	UV- visible spectrometry, Q absorbance ratio, Lamivudine, Zidovudine, Validation
Published In	Volume 7, Issue 3, May-June 2025
Published On	2025-06-17
DOI	https://doi.org/10.36948/ijfmr.2025.v07i03.47305
Short DOI	https://doi.org/g9qp4p

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