International Journal For Multidisciplinary Research

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Development of Amino Acid-Rich Protein Supplement to Stimulate Protease for Muscle Growth

Author(s) Ms. Akshatha K, Ms. Vasumati Sri P, Ms. Anit Thomas
Country India
Abstract Legumes are excellent sources of protein, carbohydrates, and minerals, and their nutritional quality can be enhanced through processing methods like soaking and cooking. Combining legumes with cereals improves their amino acid profiles, making them increasingly popular as plant-based protein options in response to dietary trends and health concerns. Bromelain, a protease from pineapples, is extensively studied for therapeutic use and can be economically sourced from pineapple waste. The present study aims to develop a protein supplement with the incorporation of bromelain to meet the requirements of amino acid post-workout for a better outcome for the muscle. The methodology included a selection of ingredients along with flavouring ingredients. The samples were pretreated by soaking, germination, and dehulling while developing protein powder consistency and its fruit flavors were adjusted. A sensory evaluation reveals that pineapple fruit flavour seems to be the most acceptable. Nutritional analysis was determined using standardized analytical techniques showing the supplements had notable protein content (26.60 g) and low fat (0.38 g). A gelatinase test verified the presence of protease enzymes in the fruit powders, highlighting their protein-breaking capabilities. The developed protein supplement with pineapple offered a well-rounded nutritional profile, improved digestion with proteolytic enzymes and received high marks in sensory tests, presenting it as an attractive and health-conscious dietary choice.
Keywords Protein supplement, Pineapple, Amino acids, Muscle build, Bromelain.
Field Sociology > Home Science
Published In Volume 7, Issue 3, May-June 2025
Published On 2025-06-09
DOI https://doi.org/10.36948/ijfmr.2025.v07i03.47474
Short DOI https://doi.org/g9pzz9
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