“Cytotoxicity assessment of Annona muricata leave extracts on (in vitro) cultured Breast tumour xenograft cells, B16F1 murine melanoma cells and cultured lymphocytes by MTT assay”

Prachi Mishra; Ankur Chhari; Sameena Akhter; Shazia Khan; Divya Patel; Sarfaraz Hanfi

doi:10.36948/ijfmr.2025.v07i04.51757

“Cytotoxicity assessment of Annona muricata leave extracts on (in vitro) cultured Breast tumour xenograft cells, B16F1 murine melanoma cells and cultured lymphocytes by MTT assay”

Author(s)	Ms. Prachi Mishra, Dr. Ankur Chhari, Dr. Sameena Akhter, Dr. Shazia Khan, Dr. Divya Patel, Mr. Sarfaraz Hanfi
Country	India
Abstract	The ongoing rise in the number of cancer cases increases the concerns regarding the efficacy of the various treatment methods that are currently available. Consequently, patients are looking for alternatives to traditional cancer treatments such as surgery, chemotherapy, and radiotherapy as a replacement. Medicinal plants are universally acknowledged as the cornerstone of preventative medicine and therapeutic practices and (Soursop) Annona muricata L is a traditional medicinal plant that has been widely used as an anticancer treatment and requires more thorough study. This research, inspired by the bioactivity from different studies of the aqueous and alcoholic extract of A. muricata, we employed it as a plant-based material for the green synthesis of metal and polymers based nanoparticles to assess their cytotoxic activity on different cultured cells by MTT assay. The synthesized NPs were characterized using double beam UV–visible spectroscopic analysis, and microscopy. The aqueous extract of A. Muricata shows higher level of cytotoxicity with lesser viable cells on each cell culture while ethanolic conjugates have shown moderate cytotoxicity and the extract of 95% methanolic have very poor cytotoxicity effect on breast cell culture as well as lymphocyte culture but have shown moderate effect on B16F1 cells. Therefore, the leaves of the medicinal plant A. muricata contained compounds that on extraction exerted an effective activity as an anticancer treatment for cancer. Our data identify graviola extracts and their constituents as promising sources for new chemo preventive and therapeutic agent(s) to be further developed for the control of breast cancer ad melenoma skin cancer. Further in vitro and in vivo studies should be carried out to explore the molecular mechanisms underlying their anticancer activity and more detail toxicity study to ensure they are safe for human consumption.
Keywords	Annona muricata L., Breast xenograft culture, B16F1 cells, MTT assay, Cell viability.
Field	Biology > Genetics / Molecular
Published In	Volume 7, Issue 4, July-August 2025
Published On	2025-07-23
DOI	https://doi.org/10.36948/ijfmr.2025.v07i04.51757
Short DOI	https://doi.org/g9t2c5

View / Download PDF File

E-ISSN 2582-2160

doi

CrossRef DOI is assigned to each research paper published in our journal.

IJFMR DOI prefix is
10.36948/ijfmr

Downloads

Research Paper Format Copyright Permission Form and Undertaking Form Cover Page Vol 7 Isu 4 Cover Page Vol 7 Isu 3 Cover Page Vol 7 Isu 2

All research papers published on this website are licensed under Creative Commons Attribution-ShareAlike 4.0 International License, and all rights belong to their respective authors/researchers.

CC-BY-SA

About IJFMR Fees & Payment Current Issue Publication Archive	Submit Research Paper Track Submission Status Publication Guidelines Publication Ethics Peer Review & Plagiarism	Join as a Reviewer Editors & Reviewers Reviewer Referral Program Get Reviewer Membership Certi.	Website/Journal Policies Usage Policy Content Policies Privacy Policy

Contact Us		+91-9687-828-838	editor@ijfmr.com

International Journal For Multidisciplinary Research

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

“Cytotoxicity assessment of Annona muricata leave extracts on (in vitro) cultured Breast tumour xenograft cells, B16F1 murine melanoma cells and cultured lymphocytes by MTT assay”

Share this