International Journal For Multidisciplinary Research

E-ISSN: 2582-2160     Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

Call for Paper Volume 7, Issue 4 (July-August 2025) Submit your research before last 3 days of August to publish your research paper in the issue of July-August.

“Cytotoxicity assessment of Annona muricata leave extracts on (in vitro) cultured Breast tumour xenograft cells, B16F1 murine melanoma cells and cultured lymphocytes by MTT assay”

Author(s) Ms. Prachi Mishra, Dr. Ankur Chhari, Dr. Sameena Akhter, Dr. Shazia Khan, Dr. Divya Patel, Mr. Sarfaraz Hanfi
Country India
Abstract The ongoing rise in the number of cancer cases increases the concerns regarding the efficacy of the various treatment methods that are currently available. Consequently, patients are looking for alternatives to traditional cancer treatments such as surgery, chemotherapy, and radiotherapy as a replacement. Medicinal plants are universally acknowledged as the cornerstone of preventative medicine and therapeutic practices and (Soursop) Annona muricata L is a traditional medicinal plant that has been widely used as an anticancer treatment and requires more thorough study.
This research, inspired by the bioactivity from different studies of the aqueous and alcoholic extract of A. muricata, we employed it as a plant-based material for the green synthesis of metal and polymers based nanoparticles to assess their cytotoxic activity on different cultured cells by MTT assay. The synthesized NPs were characterized using double beam UV–visible spectroscopic analysis, and microscopy.
The aqueous extract of A. Muricata shows higher level of cytotoxicity with lesser viable cells on each cell culture while ethanolic conjugates have shown moderate cytotoxicity and the extract of 95% methanolic have very poor cytotoxicity effect on breast cell culture as well as lymphocyte culture but have shown moderate effect on B16F1 cells. Therefore, the leaves of the medicinal plant A. muricata contained compounds that on extraction exerted an effective activity as an anticancer treatment for cancer. Our data identify graviola extracts and their constituents as promising sources for new chemo preventive and therapeutic agent(s) to be further developed for the control of breast cancer ad melenoma skin cancer. Further in vitro and in vivo studies should be carried out to explore the molecular mechanisms underlying their anticancer activity and more detail toxicity study to ensure they are safe for human consumption.
Keywords Annona muricata L., Breast xenograft culture, B16F1 cells, MTT assay, Cell viability.
Field Biology > Genetics / Molecular
Published In Volume 7, Issue 4, July-August 2025
Published On 2025-07-23
DOI https://doi.org/10.36948/ijfmr.2025.v07i04.51757
Short DOI https://doi.org/g9t2c5

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