International Journal For Multidisciplinary Research

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Isolation & Identification of Microbiota Present in Different Probiotic Samples & Test Against Commercially Available Antibiotics

Author(s) Abhijit Panda, Disha Mohanty, Dharitri Priyadarsini, Rajat Kumar Nayak, Pritismita Mahalik
Country India
Abstract Probiotic bacteria are primarily offered in fermented foods, and dairy products which plays an important role as probiotic carriers. For various reasons, functional dairy foods are highly adapted to promote probiotics' favorable health image: (i) Fermented foods, particularly dairy products, have a positive health image due to their centuries-long use; (ii) people are aware that fermented foods contain living microorganisms; and (iii) probiotics are used as a starter to combine the positive images of fermentation and probiotic cultures. Probiotics are live bacterial formulations (food or medication) that have been clinically proven to improve human health. The majority of probiotics work by regulating mucosal barrier function and immunological activity. The human digestive tract and intestinal microbiota are specialized targets and possess certain characteristics of probiotics. In this research, commercially available antibiotics and separated microorganisms from them by culturing them in various nutritional media. The antibacterial activity was then observed. Different biochemical tests have been performed on samples to identify the types of bacteria present. The bacteria identified from pickle, lassi, watermelon, soy sauce were Micrococcus varians, Pseudomonas aeruginosa, Vibrio species, Lactobacillus fermenti, respectively. The antimicrobial activity of different antibiotics was tested against the isolated microbes from which cefolac-200 shows greater zone of inhibition against Pseudomonas aeruginosa (26.75 mm) lowest zone of inhibition against Lactobacillus fermenti (11.5 mm). Azithromycin and CV-625 does not show any zone of inhibition against Micrococcus varians. Some future studies should be performed to use these isolates reliably including molecular techniques like 16S rRNA sequencing for accurate identification of lactic acid bacterial species.
Keywords Probiotics, Micrococcus varians, Pseudomonas aeruginosa, Vibrio species, Lactobacillus fermenti, cefolac-200, CV-625, Azithromycin.
Field Biology > Bio + Chemistry
Published In Volume 5, Issue 4, July-August 2023
Published On 2023-08-14
Cite This Isolation & Identification of Microbiota Present in Different Probiotic Samples & Test Against Commercially Available Antibiotics - Abhijit Panda, Disha Mohanty, Dharitri Priyadarsini, Rajat Kumar Nayak, Pritismita Mahalik - IJFMR Volume 5, Issue 4, July-August 2023. DOI 10.36948/ijfmr.2023.v05i04.5362
DOI https://doi.org/10.36948/ijfmr.2023.v05i04.5362
Short DOI https://doi.org/gsmnpn
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