International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

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A Comparative Study of Traditional and Molecular Methods for Detecting Bacterial Infections in Tertiary Care Hospitals

Author(s) Prof. Prasenjit Deb, Ms. Tanusree Rakshit
Country India
Abstract Timely and accurate detection of bacterial infections in tertiary care hospitals is essential for effective patient management and antimicrobial stewardships. Traditional clinical methods, mainly culture-based techniques, remain gold standard for identification of bacteria. After morphological and biochemical analyzes in incubation of clinical samples in these methods, antibiotic sensitivity allows the isolation of feasible pathogens for testing. However, traditional methods are labor-intensive and time taking time, often requiring several days for certain results. Polymerase chain reaction (PCR), nucleic acid amplification tests, and molecular clinical methods, including mass spectrometry, provide sensitive and unique identity directly, rapidly, rapidly from clinical samples. These techniques greatly reduce turnaround time and improve rapid, slow growing or non-cultural bacteria detection
Keywords Bacterial infection, tertiary care hospital, clinical microbiology, culture-based technology, molecular diagnosis, polymerase chain reaction, PCR, nucleic acid amplification test, NAATS, mass spectrometry, diagnostic accuracy, pathogen, pathogenic identity, turnaround time, antimicrobial bacteria, sulfin Bacteria, Slaving bacteria. Cost-effectiveness, technical expertise, infection control, outbreak detection, hybrid diagnostics, laboratory efficiency, antimicrobial stewardship, targeted therapy, public health, initial diagnosis, patient results
Field Medical / Pharmacy
Published In Volume 7, Issue 4, July-August 2025
Published On 2025-08-23
DOI https://doi.org/10.36948/ijfmr.2025.v07i04.54059
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E-ISSN 2582-2160
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