Isolation and Molecular Characterization of Bacillus cereus Strain from the Soil Samples Collected from Rhizosphere Zone of Different Crops of Uttar Pradesh India

Riazuddin Riazuddin; Vanshika Vanshika; Manish Kumar Sharma; Jagpal Singh

doi:10.36948/ijfmr.2025.v07i06.61591

Isolation and Molecular Characterization of Bacillus cereus Strain from the Soil Samples Collected from Rhizosphere Zone of Different Crops of Uttar Pradesh India

Author(s)	Mr. Riazuddin Riazuddin, Ms. Vanshika Vanshika, Mr. Manish Kumar Sharma, Mr. Jagpal Singh
Country	India
Abstract	Bacteria are a very diverse group of organisms in soil, and major taxonomic groups are represented in most soils. The extent of the diversity of microorganisms in soil is seen to be critical to the maintenance of soil health and quality, because a wide range of bacteria are involved in the important soil functions. The present study dealt with the isolation and molecular characterization of Bacillus cereus strains isolated from the field of Mustard crop in Ghaziabad at GPS location (28.6573510 Latitude 77.478410 Longitude). The total 173 soil samples were collected from rhizosphere zone of 14 different crops; Sugarcane, Pea, Lemon, Peach, Mustard, Okra, Wheat, Garlic, Alfalfa, Barley, Sorghum, Potato, Fenugreek and Mango covering 32 locations of 5 districts; Ghaziabad, Meerut, Baghpat, Hapur and Bulandshahr of Uttar Pradesh, India. The media used in this research were nutrient broth and agar. Bacillus species were identified by their phenotypic and biochemical characteristics. The colony morphology observed on Nutrient Agar were circular in shape, flat to slightly raised elevation, dull, dry to slightly mucoid in young colonies surface and Creamy white to orange colour. The results of Gram staining and 3% KOH test was Gram Positive and Negative respectively of the isolates. Based on the colony morphology, gram staining and 3% KOH test the isolate was tentatively identified as a Bacillus species. As per closest matching sequence identified through NCBI BLAST analysis and revealed that Blast analysis of the partial sequences is from the genus Bacillus within the Firmicutes in the domain bacteria with similarities between 95.84% to 99.58% and finally confirmed that the isolated bacteria closely related to the Bacillus cereus strains PQ220154.1 (95.84), PP916647.1 (99.50) and OK335864.1 (99.58%).
Keywords	Bacillus cereus, Soil, Rhizosphere, Characterization
Field	Biology > Agriculture / Botany
Published In	Volume 7, Issue 6, November-December 2025
Published On	2025-11-26
DOI	https://doi.org/10.36948/ijfmr.2025.v07i06.61591
Short DOI	https://doi.org/hbdrgx

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Isolation and Molecular Characterization of Bacillus cereus Strain from the Soil Samples Collected from Rhizosphere Zone of Different Crops of Uttar Pradesh India

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