Qualitative Phytochemical Screening and In-vitro Antioxidant Evaluation of Martynia annua Leaves Collected from Alwar District, Rajasthan, India

Kamlesh Kumar Sharma; Mahendra Kumar Jeengar; Gyan Prakash Meghwal; Dev Dutt Patel; Priyadarshi Meena

doi:10.36948/ijfmr.2025.v07i06.61644

Qualitative Phytochemical Screening and In-vitro Antioxidant Evaluation of Martynia annua Leaves Collected from Alwar District, Rajasthan, India

Author(s)	Kamlesh Kumar Sharma, Mahendra Kumar Jeengar, Gyan Prakash Meghwal, Dev Dutt Patel, Priyadarshi Meena
Country	India
Abstract	Medicinal plants are recognized as valuable reservoirs of structurally diverse bioactive compounds and continue to contribute significantly to drug discovery, traditional therapeutic systems, and modern pharmacological research. Martynia annua L. (family Martyniaceae), commonly known as “Devil’s Claw,” is widely distributed across tropical regions of India and has been traditionally employed for managing inflammatory disorders, microbial infections, and various skin-related conditions. Despite its ethnomedicinal relevance, comprehensive phytochemical and antioxidant evaluations of this species remain limited. The present study aimed to investigate the phytochemical composition and in-vitro antioxidant potential of the hydroalcoholic leaf extract of M. annua collected from the Alwar district of Rajasthan, India. Leaves were shade-dried, powdered, and extracted with a methanol–water solvent system (70:30, v/v). The crude extract was qualitatively screened using standard phytochemical assays, which confirmed the presence of alkaloids, flavonoids, phenols, tannins, saponins, and quinones, whereas glycosides were absent. Antioxidant efficacy was assessed using DPPH and ABTS radical-scavenging assays. The extract demonstrated 63.17% DPPH inhibition at a concentration of 1 mg/ml, with an IC₅₀ value of 0.79 mg/ml, indicating moderate antioxidant activity relative to ascorbic acid (IC₅₀ < 0.2 mg/ml). In the ABTS assay, the extract exhibited substantial radical-scavenging potential, achieving 76.89% inhibition within six minutes. These findings suggest that M. annua leaves possess noteworthy antioxidant constituents that may contribute to the plant’s traditional therapeutic applications. The study underscores the need for further quantitative, chromatographic, and in-vivo investigations to isolate specific bioactive molecules and elucidate their pharmacological properties.
Keywords	ABTS, Antioxidant, DPPH, Martynia annua, Phytochemicals
Field	Medical / Pharmacy
Published In	Volume 7, Issue 6, November-December 2025
Published On	2025-12-05
DOI	https://doi.org/10.36948/ijfmr.2025.v07i06.61644

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Qualitative Phytochemical Screening and In-vitro Antioxidant Evaluation of Martynia annua Leaves Collected from Alwar District, Rajasthan, India

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