International Journal For Multidisciplinary Research

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Volume 7 Issue 6
November-December 2025

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Comparison of the effect of two different buffer systems used for the estimation of serum Alkaline Phosphatase on interpretation of the test results.

Author(s) Dr. Abhay Chavda
Country India
Abstract ABSTRACT
OBJECTIVES:
The commonly used IFCC method for Alkaline Phosphatase estimation is based p-Nitrophenyl Phosphate (pNPP) substrate and either of two buffers 2-amino-2-methyl-1-propanol (AMP) or Diethanolamine (DEA), this study was carried out with the objective of comparing both buffers and to find out any significant impact on interpretation of the results.

METHODOLOGY:
This method comparison study was carried out on 75 adult patients’ serum samples including normal & abnormal liver function, samples were tested simultaneously with both kits in same analyser. Statistical analysis was done using comparison plot & calculating coefficient of comparision (r) & determination (r2), comparison was done by paired t test & Bland Altmann plot.

RESULTS:
The data is distributed normally as shown by Shapiro Wilk p-value 0.52 (α=0.05). Mean & SD with AMP & DEA buffers were 125.95 +/-81.35 U/L & 260.12 +/-206.39U/L respectively. Pearson’s correlation coefficient (r) 0.9936 & coefficient of determination (r2) 0.9872 shows very strong direct relationship in the results. In paired t test, t is 9.22 & p is<0.0001shows significant difference in the results with both the buffers. Bland–Altman plot shows proportional bias ie the difference in the results increases with higher values. We derived a conversion factor of 2.06 to convert the values obtained from one buffer to another.

CONCLUSION :
This study concludes that both the buffers cannot be used interchangeably and switching over will require either a different reference range or use of a conversion factor which every lab should derive for themselves to avoid false interpretation of patients results.
Field Biology > Bio + Chemistry
Published In Volume 7, Issue 6, November-December 2025
Published On 2025-11-29
DOI https://doi.org/10.36948/ijfmr.2025.v07i06.62078
Short DOI https://doi.org/hbdsnn
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