Histopathology Of Skeletal Muscles and Liver Of  9 Months Old Labeo rohita (Hamiltin 1822) Fed With Aqua Fix During Aeromoniasis

Ankamma N; Viveka Vardhani V

Histopathology Of Skeletal Muscles and Liver Of 9 Months Old Labeo rohita (Hamiltin 1822) Fed With Aqua Fix During Aeromoniasis

Author(s)	Dr. Ankamma N, Prof. Viveka Vardhani V
Country	India
Abstract	The impact of phytogenic immunostimulant, Aqua Fix was studied on the histopathological changes in skeletal muscles and liver of L. rohita fed with Aqua Fix during aeromoniasis. Three groups (A,B ,C) of nine months old fish were used in this experiment; groups A and B were treated with Aqua Fix for 4 days, on day 5 fish of group B and C were infected with Aeromonas hydrophila @ 10-6CFU/fish(fish of group C were fed with normal diet for 4 days).Controls (group D) were untreated and uninfected. Microscopic examination of the skeletal muscle and liver tissues were carried out on selected days of experimentation (day 1 and day 4). The stained sections were observed under microscope and histological changes were noted. Immunostimulated fish (group A) showed compactly arranged long, unbranched muscle fiber bundles compared to other groups (B and C) of fish. Immunostimulated and infected fish (group B) showed derangement of bundles at certain places, lacunae and odema. Infected fish (group C) showed remarkable changes in the tissue sections. Muscle fibers underwent degeneration and broken completely. The fragments of muscle fibers and lesions were clearly visible. Both the muscle fibers and muscle bundles were healthy in controls (Group D). The liver of immunostimulated fish (group A) showed normal architecture as in control fish. Fish treated with Aqua Fix and infection (group B) showed congestion of central vein and infiltration of cells. Infected fish (group C) showed marked dilation of hepatic vein, haemorrhagic spots, pycnosis of hepatacyte nuclei, necrosis of hepatocytes, inflammation and marked vacuolation at some places. The fish (group B) fed with immunostimulant and infection exhibited moderate effect on skeletal muscle and liver suggesting that Aqua Fix supplementation supports recovery during aeromoniasis.
Keywords	Aqua Fix , Aeromoniasis , Labeo rohita , Skeletal muscle, Liver
Field	Biology > Zoology
Published In	Volume 7, Issue 6, November-December 2025
Published On	2025-12-02

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Histopathology Of Skeletal Muscles and Liver Of 9 Months Old Labeo rohita (Hamiltin 1822) Fed With Aqua Fix During Aeromoniasis

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