International Journal For Multidisciplinary Research

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A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

Call for Paper Volume 8, Issue 2 (March-April 2026) Submit your research before last 3 days of April to publish your research paper in the issue of March-April.

Evaluation of Extracellular Enzyme Activity of Azotobacter spp. in Relation to Plant Growth-Promotion

Author(s) Sejal Rupareliya, Rachana Shukla
Country India
Abstract Azotobacter spp., a group of free-living nitrogen-fixing bacteria, play a pivotal role in sustainable agriculture by enhancing soil fertility and promoting plant growth through multiple mechanisms. This study investigates the plant growth-promoting traits of Azotobacter isolates, including their ability to secrete extracellular enzymes such as amylase, lipase, protease, cellulase, chitinase, catalase, and ACC deaminase, as well as produce phytohormones, fix atmospheric nitrogen, production of siderophore, and mineralization of Phosphate. Isolates were obtained from the garden soil of the Patan region of Gujarat, India. They were characterized using morphological, microscopical, biochemical, and screening techniques. Enzyme activity was quantified using plate assays, and the enzyme index was calculated to assess their hydrolytic potential. The results demonstrated significant variability among strains, with some exhibiting high enzymatic activity and notable effects. Lastly, one potent isolate, PGY11, was selected for molecular identification and identified as Azotobacter vinelandii. These findings underscore the potential of Azotobacter spp. as bioinoculants for improving crop productivity and soil health, particularly under stress conditions.
Keywords Azotobacter spp., extracellular activity of amylase, lipase, protease, cellulase, chitinase, catalase, and ACC deaminase enzymes, and PGP traits.
Field Biology > Agriculture / Botany
Published In Volume 8, Issue 1, January-February 2026
Published On 2026-02-24

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