International Journal For Multidisciplinary Research

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Comparative Study on the Antibacterial potential of Leaf and Leaf-Derived Callus Extracts of Simarouba glauca DC.

Author(s) Prof. Dr. Sindhu K K, Ms. Shilpa S, Ms. Diya Joseph
Country India
Abstract Background: Simarouba glauca, an exotic species, is a plant known for its significant medicinal properties. Aim: The study showed the antibacterial activity of leaf-derived callus extract and leaf extract of Simarouba glauca. The objective is to develop a faster and more reliable in-vitro callus induction method for S. glauca using leaves as explants, and to compare the antibacterial activity of leaf and callus extracts. Method: Callus induction was evaluated using MS medium supplemented with different growth regulators. MS medium with Different hormonal combinations was used for the in vitro induction of callus. The antibacterial activity of Petroleum ether, Chloroform, and Acetone extracts of leaf and callus extracts of S. glauca was tested against the five different bacterial strains. The bacterial strains tested include B. subtilis, S. typhi, S. aureus, E. coli, and V. cholera. Result: MS medium with 2,4-D (5 mg/L) proved best callus induction for leaf explants, yielding maximum fresh and dry weights. Maximum zone of inhibition was observed in the Petroleum ether leaf extract against the E. Coli bacterial strain, followed by the acetone leaf extract against E. coli. In the case of antibacterial activity of callus extract, Acetone showed maximum zone of inhibition against E. coli strain, followed by Petroleum ether callus extract against B. subtilis. Conclusion: This study successfully developed an effective method for in-vitro callus induction in S. glauca and confirmed the antibacterial potential of both leaf and callus extracts.
Keywords Simarouba glauca, in-vitro callus induction, antibacterial activity, plant extracts.
Field Biology > Agriculture / Botany
Published In Volume 7, Issue 6, November-December 2025
Published On 2025-12-24
DOI https://doi.org/10.36948/ijfmr.2025.v07i06.64296
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