International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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Extraction and Isolation Techniques for Natural Products: A Comprehensive Review.”

Author(s) Ms. Priyanka Dineshkumar Yadav, Mr. Harshil Dhameliya, Mr. Avi Kakdiya, Dr. Riddhi Trivedi
Country India
Abstract It summarizes recent advances in extraction and isolation techniques for natural products, with emphasis on green and sustainable approaches. It discusses the shift from conventional solvent intensive methods, such as maceration and Soxhlet extraction, to modern techniques including ultrasound-, microwave- and supercritical fluid–assisted extraction that improve efficiency and reduce toxic solvent use. Particular focus is placed on deep eutectic solvents (DES) and natural deep eutectic solvents (NADES) as tunable, low-toxicity media for recovering bioactive phytonutrients like terpenoids, alkaloids, and polyphenols. It also covers solid–liquid extraction optimization, chromatographic separation (LPLC, preparative HPLC, TLC, GC), and complementary purification operations such as recrystallization and trituration for obtaining analytical-grade isolates. Analytical characterization using HPLC, LC–MS, NMR, IR and UV–Vis is outlined to confirm purity and structural identity of isolated compounds. Case studies, including bakuchiol, Artemisinin and paclitaxel, illustrate how optimized extraction and isolation workflows translate into successful pharmaceutical products. Finally, the article highlights current challenges in scalability, reproducibility and cost, and points to future integration of automation, microfluidics and artificial intelligence to design smarter, more sustainable extraction processes and accelerate natural product drug discovery.
Keywords Extraction, Isolation, Chromatography, HPLC, Spectroscopic, HPLC, LC–MS, NMR, IR, UV–Vis, Green technologies.
Published In Volume 8, Issue 1, January-February 2026
Published On 2026-02-06
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E-ISSN 2582-2160
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