Anti-Inflammatory Potential of Nano-Liposomal Myricetin by Protein Denaturation Assay and Its Pro-Apoptotic Effect Assessed by Flow Cytometry

Author(s)	Usha Verma, Dr. Pankaj Motilal Chaudhari
Country	India
Abstract	A naturally occurring flavonoid present in many fruits and vegetables, myricetin has garnered a lot of attention due to its possible anticancer effects. Heat-induced denaturation of bovine serum albumin was used to measure anti-inflammatory activity, and histopathological analysis was used to measure hepatoprotective effects. Furthermore, flow cytometry was used to assess myricetin's capacity to cause apoptosis. Protein denaturation was significantly inhibited by nano-liposomal myricetin in a concentration-dependent manner, with 59.1% inhibition at 100 µM, which is comparable to diclofenac sodium. Apoptotic cell populations increased dose- and time-dependently, reaching 75% at the highest concentration of 198 µM after 24 hours, according to flow cytometric analysis. These results demonstrate the potential of nano-liposomal myricetin as a natural therapeutic agent against hepatocellular carcinoma and inflammation-associated liver disorders by effectively inhibiting protein denaturation and promoting programmed cell death.
Keywords	Myricetin, Nano-liposomes, Anti-inflammatory activity, Protein denaturation, Hepatocellular carcinoma, Apoptosis
Field	Medical / Pharmacy
Published In	Volume 4, Issue 4, July-August 2022
Published On	2022-08-06
DOI	https://doi.org/10.36948/ijfmr.2022.v04i04.68711

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