International Journal For Multidisciplinary Research

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Waste to Wealth: Bioconversion of Agro-Wastes for the Production of Industrially Valuable Enzyme Tannase

Author(s) Ms. Shajitha G., Ms. Sakila S.
Country India
Abstract Agro-industrial fruit processing generates large quantities of peel wastes that pose serious environmental challenges. However, these residues are rich in tannins and other bioactive phytochemicals, making them suitable substrates for microbial enzyme production. The present study explores a waste-to-wealth approach through the bioconversion of selected fruit peel agro-wastes for the production of the industrially important enzyme tannase (tannin acyl hydrolase, EC 3.1.1.20). Aqueous extracts of fruit peels including pomegranate, grape, banana, papaya, watermelon, muskmelon, orange, and sapota were subjected to qualitative phytochemical screening to identify tannins and related constituents. The results revealed the presence of alkaloids, glycosides, carbohydrates, tannins, saponins, and flavonoids in varying combinations, confirming the suitability of these wastes as tannase-inducing substrates. Fungal isolates obtained from fruit peel waste dumping sites were screened for tannase activity using tannic acid agar medium, and efficient tannase-producing strains were identified based on clear zone formation. Among the substrates evaluated, grape peel supported the highest tannase production, followed by pomegranate peel, which may be attributed to their higher tannin and polyphenolic content. The findings of this study demonstrate that fruit peel agro-wastes can be effectively utilized as low-cost substrates for microbial tannase production, offering an eco-friendly and economically viable strategy for waste management and industrial enzyme production.
Keywords Tannase, Phytochemicals, Fruit peels
Field Biology
Published In Volume 8, Issue 1, January-February 2026
Published On 2026-02-13
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E-ISSN 2582-2160
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