Molecular Authentication of Bamboo Species Using rbcL and matK Gene-Based DNA Barcoding Approach

Author(s)	Ms. Praveena C, Franklin Charles Jose
Country	India
Abstract	Abstract Accurate taxonomic identification of bamboo species is often complicated due to morphological similarities and infrequent flowering patterns. DNA barcoding has emerged as a reliable molecular tool for species authentication. The present study aimed to molecularly characterize bamboo species using chloroplast gene regions rbcL and matK. Genomic DNA was isolated using CTAB method, amplified through PCR, and sequenced. BLAST analysis revealed 100% query coverage and E-values of 0.0 for both markers. The rbcL sequence showed 100% identity with Bambusa burmanica and 99.83% similarity with closely related taxa including Gigantochloa albociliata, Dendrocalamus membranaceus, and Bambusa arnhemica. The matK region exhibited 99.88% similarity with Bambusa burmanica, Bambusa vulgaris, Bambusa dolichomerithalla and related taxa. The combined marker analysis confirms the effectiveness of rbcL and matK as core barcoding markers for bamboo species identification.
Keywords	Keywords: DNA barcoding, rbcL, matK, Bamboo, Molecular identification, Chloroplast genes, Bambusoideae
Field	Biology > Genetics / Molecular
Published In	Volume 8, Issue 1, January-February 2026
Published On	2026-02-26

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