International Journal For Multidisciplinary Research

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Histochemical Localization of Polysaccharides During Pollinium Development in Cottonia peduncularis (Lindl.) Rchb.f. (Orchidaceae)

Author(s) Ms. Pratiksha Vithal Kamble, Kiran P Kolkar
Country India
Abstract Cottonia peduncularis (Lindl.) Rchb.f., is monotypic and rare epiphytic orchid endemic to peninsular India. The present study investigates the histochemical localization of total insoluble polysaccharides during pollinium development and relates these findings to its adaptive morphology and reproductive ecology. Flower buds at various developmental stages, collected from natural habitat and were fixed in FAA, sectioned (6-8 µm), and subjected to Periodic Acid–Schiff’s (PAS) staining following standard microtechniques. Both early and late sporogenous cells exhibited strong PAS reactivity, indicating high polysaccharide accumulation. Meiocytes retained PAS-positive reactions in cytoplasm and nucleoli throughout meiosis, followed by the formation of inseparable tetrads through successive cytokinesis. A progressive increase in PAS positivity was observed in the cytoplasm of tetrads and microspores, reaching maximum intensity in the two-celled stage, particularly in the pollen wall. The tapetum is the secretory type, showed strong PAS reaction and gradual degeneration during tetrad formation, contributing polysaccharide-rich material to developing pollen. These results suggest the essential role of carbohydrates as an energy source during meiosis, wall differentiation, and pollen maturation. The findings provide insight into the biochemical basis of reproductive adaptation in C. peduncularis, linking its histochemical dynamics with ecological and evolutionary specialization within the Orchidaceae.
Keywords Orchids, Pollinia, Histochemistry, Polysaccharides.
Field Biology > Agriculture / Botany
Published In Volume 8, Issue 1, January-February 2026
Published On 2026-02-27
DOI https://doi.org/10.36948/ijfmr.2026.v08i01.70067
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