International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

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Volume 8 Issue 2
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Production of Eco-friendly Liquid Detergent with Modified Microbial Enzymes

Author(s) Prof. Dr. Savanta V. Raut, Mr. Jay Kanaiyalal Panchal
Country India
Abstract The environmental impact of conventional chemical detergents has increased the demand for sustainable and biodegradable alternatives. In the present study, microbial hydrolytic enzymes were explored for the development of an eco-friendly liquid detergent formulation. Bacterial strains producing amylase, protease, and lipase were isolated from environmentally stressed habitats and screened using substrate-specific media. Selected isolates were cultured for enzyme production, and crude enzymes were partially purified by ammonium sulphate fractionation. The enzymes were further modified through polysaccharide–aldehyde conjugation to improve stability and detergent compatibility. The modified enzymes exhibited enhanced stability and retained higher relative activity in the presence of detergent formulation components, including surfactants, builders, viscosity modifiers, and preservatives, compared to unmodified enzymes. An enzyme-based liquid detergent was formulated using the modified enzymes and evaluated for cleaning efficiency by visual stain removal analysis. The formulated detergent demonstrated effective removal of common household stains and showed performance comparable to a commercial detergent, while water served as a negative control. The findings indicate that modified microbial hydrolytic enzymes can be effectively utilized in eco-friendly liquid detergent formulations, offering a sustainable alternative to conventional detergents and contributing to environmentally responsible cleaning technologies
Keywords Microbial enzymes; Amylase; Protease; Lipase; Enzyme modification; Eco-friendly detergent; Liquid detergent formulation
Published In Volume 8, Issue 2, March-April 2026
Published On 2026-03-11
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E-ISSN 2582-2160
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