Assessing the Safety and Efficacy of a Biotherapeutic Product for Treating Wounds Using Homologous Animal Model

Senthilkumar Natesan; Sanjana Shah

Assessing the Safety and Efficacy of a Biotherapeutic Product for Treating Wounds Using Homologous Animal Model

Author(s)	Dr. Senthilkumar Natesan, Ms. Sanjana Shah
Country	India
Abstract	Proliferation, inflammation, and tissue remodeling are stages in the intricate biological process of wound healing. The assurance of autologous cell therapy, which uses a patient's own cells to stimulate tissue regeneration and improve healing outcomes has been brought to light by recent developments in regenerative medicine. This study used chickens as a preclinical homologous wound healing model to assess a new autologous cell treatment technique. Chicken’s autologous peripheral blood-derived mononuclear cells were separated and their appearance and functional characteristics were examined. Their migratory capacity a crucial component for tissue regeneration and wound healing was evaluated using a scratch test. Cell survival and traits similar to human therapeutic cells were further verified by functional and phenotypic investigations. Each bird had excision wounds made in order to assess the effectiveness of the treatment; one wound was treated with the autologous cell preparation, and the other wound was left untreated as an internal control. Over the course of ten days, the progress of wound healing was tracked. With an average wound area reduction of 92% by day 10, the treated wounds showed noticeably faster healing than the untreated control wounds, which showed a reduction of 56.75% (p = 0.019). Because of their similar immunological and healing systems, the study emphasizes chickens as a viable, moral, and economical substitute. This study demonstrates that the novel autologous cell therapy is both safe and effective in a homologous chicken wound model. It also highlights the affordability and translational applicability behind using chicken model for assessing cell-based therapies with the potential of autologous cell therapy to promote tissue regeneration and better healing outcomes.
Keywords	Wound healing, Autologous cell therapy, Process validation, Chicken model
Field	Biology > Medical / Physiology
Published In	Volume 8, Issue 2, March-April 2026
Published On	2026-03-17

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Assessing the Safety and Efficacy of a Biotherapeutic Product for Treating Wounds Using Homologous Animal Model

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