Protoplast Isolation and Fusion in Amomum Subulatum Roxb

Author(s)	Kul Bahadur Subba, Arundhati Bag, B. C. Basistha
Country	India
Abstract	Amomum subulatum Roxb. is an important spice and cash crop of Himalayan region of Indian sub-continent. An attempt has been made to isolate and fuse protoplast from the in-vitro grown seedling of Amomum subulatum Roxb. Off the various enzyme combination tried, the one containing the enzyme combination of 2% cellulase “Ozonuka” RS (Yakult Pharmaceutical Ind. Co. Ltd, Japan) 1% Macerozyme R-10 (Yakult Pharmaceutical Ind. Co. Ltd, Japan) and 1 % Hemi-cellulase (Sigma-Aldrich, USA) prepared in 10.93% D- Mannitol (Himedia, India) and 0.055% calcium chloride yielded protoplast of 2 x 104/g of leaf tissue. Protoplast fusion was achieved using 20% Polyethylene Gylcol (PEG 4000) but in small quantity added to the mixed protoplast. To develop a successful somatic hybrid of commercial importance further research is required.
Keywords	Protoplast, enzymes, cell wall digestion, protoplast fusion, somatic hybridization, Poly ethylene glycol
Field	Biology > Agriculture / Botany
Published In	Volume 5, Issue 5, September-October 2023
Published On	2023-10-11
Cite This	Protoplast Isolation and Fusion in Amomum Subulatum Roxb - Kul Bahadur Subba, Arundhati Bag, B. C. Basistha - IJFMR Volume 5, Issue 5, September-October 2023. DOI 10.36948/ijfmr.2023.v05i05.7403
DOI	https://doi.org/10.36948/ijfmr.2023.v05i05.7403
Short DOI	https://doi.org/gsv6h4

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