International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

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Volume 8 Issue 3
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Impact of Fiber Morphology on the Degradation of Salivary Amylase Using Starch-Iodine Indicators

Author(s) Ms. Theja Shree R, Karthik Jayakara Shetty
Country India
Abstract Saliva is a frequently encountered biological fluid in forensic casework, with alpha amylase serving as its primary biochemical marker. The starch iodine test remains one of the simplest presumptive methods for saliva detection, yet its reliability is strongly influenced by the substrate on which saliva is deposited. This study examines the impact of fibres morphology on the degradation of salivary amylase using starch iodine indicators. Cotton, semi cotton, rayon, and lycra fabrics were selected to represent natural, blended, and synthetic substrates. Saliva samples were applied to each fibres type and monitored for enzymatic activity through characteristic colorimetric changes. Results revealed that porous natural fibres such as cotton retained amylase activity longer, producing sustained starch hydrolysis, while synthetic fibres like lycra demonstrated rapid enzyme loss and weaker reactions. These findings highlight the critical role of fibres structure and diffusion dynamics in forensic detection, emphasizing that substrate choice can affect presumptive test outcomes and subsequent DNA recovery. The study contributes to forensic serology by linking textile morphology with biochemical persistence, offering practical insights for crime scene investigations involving saliva evidence.
Keywords Salivary amylase, starch iodine test, fibres morphology, forensic serology, textile substrates
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-05-09
DOI https://doi.org/10.36948/ijfmr.2026.v08i03.77415
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E-ISSN 2582-2160
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