International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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Volume 8 Issue 3
May-June 2026

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Functionalization of Metal and Metal Oxide Nanoparticles for Enzyme Immobilization

Author(s) Dr. Heena Devnani, Dr. Unnati Dani
Country India
Abstract The enzymes lipase and urease were immobilized on inorganic nanoparticles by the crosslinking method using glutaraldehyde. The nanoparticles were purchased commercially and used without any further modifications. Lipase activity was measured by titrimetric method whereas Urease activity was calculated using Nessler’s reagent method, in free as well as immobilized state. Activity as a function of pH, temperature, storage stability, reusability and Km was analyzed and their results were compared. The optimum pH range of free lipase, 7.2 to 7.4 showed an increase in the range from 7 to 8 when immobilized. Similarly, in thermal regulation optimum temperature range for free lipase was increased on immobilization. The immobilized lipase showed better storage stability as compared to free lipase, which was maximum for lipase on zinc oxide nanoparticles. A similar trend was observed in the activities of immobilized urease. The results clearly depict the beneficial effects of immobilization. Further, it revealed zinc oxide nanoparticles to be the best support material, followed by silver nanoparticles and lastly aluminium oxide nanoparticles. Kinetic studies were performed to confirm these outcomes
Keywords Enzyme immobilization, inorganic nanoparticles, optimization, reusability, enzyme kinetics.
Field Biology
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-05-07
DOI https://doi.org/10.36948/ijfmr.2026.v08i03.77523
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E-ISSN 2582-2160
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