International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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Impact on Creatinine Detection from Urine Stains of Different Fabric Surfaces for Forensic Identification using Jaffe’s Method

Author(s) Mr. Rogash Snowin M, Ms. Akshara M P
Country India
Abstract The identification of urine stains at crime scenes plays a crucial role in forensic investigations, particu-larly in cases where other biological evidence is absent. This study evaluated the effect of different fab-ric surfaces on the persistence and detectability of creatinine in urine stains using Jaffe’s Method. Five commonly encountered fabrics such as cotton, rayon, silk, polyester, and chiffon were selected for analysis. Equal quantities of urine samples were deposited on each fabric and allowed to dry under con-trolled environmental conditions. The persistence study was conducted for 40 days at fixed intervals to determine the detectability of creatinine over time. Jaffe’s reaction was used for identification, where creatinine reacts with alkaline picric acid to produce an orange-red coloured complex. The findings re-vealed that creatinine detectability gradually decreased with time on all fabrics; however, the retention capacity varied significantly depending on the fabric type. Rayon showed the highest persistence with minimal fading even after 40 days, whereas polyester exhibited rapid loss of detectability. Cotton demonstrated moderate persistence, while silk and chiffon showed reduced retention with progressive fading after 20 days. The study concludes that both fabric type and aging period significantly influence creatinine detection in urine stains. These findings are important for forensic investigations involving aged biological stains and support the need for substrate-specific interpretation during urine stain anal-ysis.
Keywords Creatinine Detection, Urine Stains, Jaffe Reaction, Fabric Persistence, Forensic Identification, Cotton, Rayon, Polyester
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-05-13
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E-ISSN 2582-2160
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