International Journal For Multidisciplinary Research

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Call for Paper Volume 8, Issue 3 (May-June 2026) Submit your research before last 3 days of June to publish your research paper in the issue of May-June.

Analytical Standardization and Fatty Acid Profiling of Brahmi Ghrita: A Physicochemical Evaluation of a Classical Ayurvedic Medicated Ghee

Author(s) Dr. Monika Kaushik, Dr. Bharat Sethi, Prof. Dr. Priyanka Wadhwa, Dr. Sakshi Choudhary
Country India
Abstract Background
Brahmi Ghrita is a classical Ayurvedic medicated ghee widely used as a Medhya Rasayana for cognitive and neurological disorders. Standardization and analytical evaluation are essential to ensure the quality, purity, and therapeutic reliability of herbal formulations.
Objective
To evaluate the physicochemical parameters and fatty acid profile of prepared Brahmi Ghrita through analytical standardization.
Materials and Methods
Brahmi Ghrita was prepared according to the guidelines of the Ayurvedic Formulary of India (AFI) in the Rasashastra Department under expert supervision. The prepared sample was analyzed at Patanjali Research Foundation, Haridwar (Report No. PRF/2025/0029). Physicochemical parameters including acid value, peroxide value, iodine value, saponification value, refractive index, and density were assessed. Fatty acid profiling was carried out using GC-FID analysis.
Results
The analytical study showed acid value 0.37, peroxide value 0.95 meq/kg, iodine value 19.86, saponification value 357.83, refractive index 1.4618, and density 0.8919 g/mL, indicating good stability and low oxidative degradation. GC-FID analysis identified major fatty acids including palmitic acid (29.68%), stearic acid (9.88%), myristic acid (9.22%), oleic acid (8.79%), linoleic acid (2.08%), and butyric acid (2.82%).
Conclusion
The analytical parameters confirmed the quality and stability of Brahmi Ghrita. The presence of biologically important fatty acids supports its therapeutic potential and validates its classical Ayurvedic utility.
Keywords Brahmi Ghrita, Analytical Standardization, Physicochemical Analysis, GC-FID, Fatty Acid Profile, Ayurvedic Formulation
Field Medical / Pharmacy
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-05-17

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