International Journal For Multidisciplinary Research

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International Journal For Multidisciplinary Research

Author(s) Mr. Vivek Yadav, Prof. Dr. Anita singh, Dr. Teena Agarwal
Country India
Abstract Soil microbial communities play a critical role in maintaining ecosystem stability and nutrient cycling. In the present study, a total of 40 bacterial isolates were obtained from soil samples and characterized using morphological, microscopic, and biochemical approaches. Colony morphology revealed significant diversity in terms of size (1–5 mm), shape (circular and irregular), pigmentation (off-white, creamy, and orange), margin (entire, undulate, lobate), and elevation (flat, raised, umbonate).Microscopic analysis indicated that the majority of isolates were Gram-positive rods, while a few exhibited cocci morphology. Most isolates were motile, suggesting active adaptation in soil environments.Biochemical characterization demonstrated metabolic variability among isolates. Differential responses were observed in catalase activity, citrate utilization, carbohydrate fermentation (glucose, lactose, sucrose), MR-VP tests, and indole production, indicating diverse metabolic pathways.The results highlight the presence of a functionally diverse and metabolically versatile bacterial community, emphasizing their potential role in soil fertility, nutrient cycling, and plant growth promotion.
Keywords Soil bacterial isolates; Semi-arid soils; Morphological characterization; Biochemical characterization; Rajasthan; Microbial diversity; Soil health; Sustainable agriculture.
Field Biology > Agriculture / Botany
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-05-29
DOI https://doi.org/10.36948/ijfmr.2026.v08i03.79715
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E-ISSN 2582-2160
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