International Journal For Multidisciplinary Research

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To Evaluate the Antidiabetic Potential of Clerodendrum Infortunatum, (Bhat) Leaf and Saraca Asoca Bark Extracts through Phytochemical Analysis

Author(s) Mr. Ashok Kumar Priyadarshi, Dr. Satendra Kumar
Country India
Abstract Background: Diabetes mellitus is a chronic metabolic disorder affecting over 537 million adults globally, with India bearing the second-highest burden worldwide. The inadequacy and adverse-effect profiles of current antidiabetic drugs have intensified the search for plant-derived therapeutic alternatives. Clerodendrum infortunatum L. (Lamiaceae), commonly known as Bhat or Glorybower, and Saraca asoca (Roxb.) Willd. (Leguminosae), known as Ashoka, are extensively used in traditional Ayurvedic and folk medicine systems of India. Both species have been ethnopharmacologically documented for the management of metabolic disorders, yet rigorous phytochemical-pharmacological correlative data for antidiabetic applications remain limited. Objective: The present study aimed to perform comprehensive phytochemical profiling of C. infortunatum leaf and S. asoca bark extracts, quantify major bioactive classes, and evaluate their in vitro antidiabetic potential through α-amylase and α-glucosidase inhibition assays, as well as glucose uptake stimulation in L6 myotubes. Methods: Successive solvent extractions were performed using petroleum ether, chloroform, ethyl acetate, methanol, and aqueous solvents. Qualitative phytochemical screening, HPTLC fingerprinting, HPLC-DAD quantification, and LC-MS/MS characterization were employed. Antidiabetic activity was assessed via enzyme inhibition (α-amylase IC₅₀, α-glucosidase IC₅₀) and pancreatic β-cell protection assay. Results: The methanol extract of C. infortunatum leaf (MECI) demonstrated the highest total phenolic content (168.4 ± 2.9 mg GAE/g) and total flavonoid content (86.2 ± 1.8 mg QE/g). The methanol extract of S. asoca bark (MESA) recorded TPC of 192.6 ± 3.4 mg GAE/g and TFC of 78.4 ± 1.6 mg QE/g. LC-MS/MS identified 19 major compounds across both species including catechin, epicatechin, quercetin, apigenin, luteolin, gallic acid, and β-sitosterol. MECI exhibited α-amylase IC₅₀ of 84.6 ± 2.2 µg/mL and α-glucosidase IC₅₀ of 72.4 ± 1.9 µg/mL. MESA showed α-amylase IC₅₀ of 68.2 ± 1.8 µg/mL and α-glucosidase IC₅₀ of 54.8 ± 1.4 µg/mL, compared to acarbose (α-amylase IC₅₀ 42.4 ± 1.2 µg/mL; α-glucosidase IC₅₀ 38.6 ± 0.9 µg/mL). Conclusion: Both plant extracts demonstrate significant antidiabetic potential through enzyme inhibition mechanisms, with S. asoca bark showing superior potency. The findings provide a strong scientific basis for further in vivo validation and drug development.
Keywords : Clerodendrum infortunatum; Saraca asoca; Antidiabetic; Phytochemical screening; α-Amylase inhibition; α-Glucosidase inhibition; Flavonoids; Phenolics; HPLC; LC-MS/MS
Field Chemistry > Pharmacy
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-06-10
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E-ISSN 2582-2160
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CrossRef DOI prefix of IJFMR is 10.36948/ijfmr

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