International Journal For Multidisciplinary Research

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Detection of Dengue, Chikungunya and Zika Viruses using Single-reaction Multiplex Real-time RT-PCR Assay in Patients of Acute Febrile Illness

Author(s) Dr. Areet Bhangu, Dr. Shailpreet Kaur Sidhu, Dr. Kanwardeep Singh, Dr. Loveena Oberoi, Dr. Ratneev Kaur
Country India
Abstract OBJECTIVE : Dengue, Chikungunya and Zika viruses are a major public health problem worldwide. The global distribution of these viruses represents a challenge for clinicians due to the similarities between their manifestations and overlapping symptoms. The present study was carried out to detect Dengue, Chikungunya and Zika viruses from patients of acute undifferentiated febrile illness and also to determine the co-infection of Dengue and Chikungunya viruses and to determine the circulating serotypes (1-4) of Dengue virus.
METHODS : The present study was conducted in the Viral, Research and Diagnostic Laboratory, Department of Microbiology, Government Medical College, Amritsar from a period of 18 months w.e.f. from 1st July 2024 to 31st December 2025. A total of 400 suspected cases with acute febrile illness were investigated for the detection of Dengue, Chikungunya and Zika viruses using Single – Reaction Multiplex Real-Time RT-PCR.
RESULTS : Out of 400 cases 31.75% were tested positive for viral infections. Chikungunya (16%) and Dengue (15.75%) were almost equally detected, while no case of Zika virus was detected.

CONCLUSION : The study highlights the importance of multiplex real-time RT-PCR as a powerful diagnostic tool for the simultaneous detection and differentiation of major arboviral pathogens responsible for febrile illness.
Keywords Dengue, Chikungunya, Co-infection, Arboviruses, Dengue Serotype.
Field Medical / Pharmacy
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-06-10
DOI https://doi.org/10.36948/ijfmr.2026.v08i03.81054
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