International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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Volume 8 Issue 4
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Analysis of Phytochemical Constituents and Antibacterial Properties of Dalbergia sissoo Leaf Extract from Rajasthan

Author(s) Ms. Priyanka Vaishnav, Kishor Singh
Country India
Abstract The excessive use of chemical antimicrobial agents has resulted in serious environmental and human health concerns, particularly the rapid appearance of drug-resistant microorganisms. Therefore, medicinal plants have gained significant interest as natural sources of bioactive antimicrobial compounds. The current study aims to analyze the phytochemical properties and antibacterial activity of leaf extracts of Dalbergia sissoo, a medicinal plant frequently employed in folk herbal medicine. Leaves of the plant were subjected to extraction via decoction and maceration methods by using different solvents such as petroleum ether, hexane, chloroform, methanol, ethyl acetate, and water. The initial phytochemical study showed that the plant possessed flavonoids, phenols, alkaloids, tannins, glycosides, saponins, steroids, and terpenoids, and the methanolic extract demonstrated more phytochemical constituents. Antibacterial activity was determined using crude and fractionated extracts against four bacteria: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae via well diffusion and disc diffusion methods. The extracts showed concentration-dependent antibacterial activity, producing low to moderate zones of inhibition, with methanolic and maceration extracts demonstrating comparatively higher efficacy. Staphylococcus aureus showed maximum susceptibility, while Escherichia coli showed low susceptibility. Although it showed less effect than kanamycin, the extract demonstrated some antibacterial activity; however, no inhibition was observed in the DMSO control, indicating that the effect was due to the presence of active compounds in the extract. This study validates the use of a locally available ethnomedicinal plant in the region through scientific research.
Keywords Dalbergia sissoo, phytochemical analysis, antibacterial activity, medicinal plants, solvent extracts
Field Biology > Agriculture / Botany
Published In Volume 8, Issue 3, May-June 2026
Published On 2026-06-11
DOI https://doi.org/10.36948/ijfmr.2026.v08i03.81154
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CrossRef DOI prefix of IJFMR is 10.36948/ijfmr

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