International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

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Volume 8 Issue 4
July-August 2026

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Conjugation: A Preliminary Study of Recombinant DNA Technology (RDT)

Author(s) Dr. Sohni Jain, Mr. Krishna Singh Raghuvanshi
Country India
Abstract The present study investigates bacterial conjugation as a key mechanism of horizontal gene transfer and its relevance to Recombinant DNA Technology (RDT). Conjugation enables the transfer of plasmids between bacterial cells through direct contact, mediated by the tra gene and the Type IV secretion system. Using Escherichia coli as a model organism, this experiment demonstrates the transfer of tetracycline resistance from an F+ donor to an F- recipient strain. The process was carried out using a specialized GeNie conjugation kit, followed by antibiotic screening on Luria Bertani agar media supplemented with tetracycline and streptomycin. Growth observed on dual antibiotic plates confirmed successful plasmid-mediated transfer of the resistance gene, validating the occurrence of conjugation. The findings are consistent with earlier reports describing conjugative plasmid transfer as a major contributor to antibiotic resistance dissemination. Beyond its biological significance, the study highlights the application of conjugation in genetic engineering and molecular cloning, where it serves as a foundational process for DNA manipulation. Overall, this research provides both theoretical and experimental understanding of bacterial conjugation, emphasizing its role in evolution, antibiotic resistance spread, and biotechnological applications.
Keywords Bacterial conjugation, Escherichia coli, horizontal gene transfer, antibiotic resistance, recombinant DNA technology, plasmid.
Field Biology > Genetics / Molecular
Published In Volume 8, Issue 4, July-August 2026
Published On 2026-07-05
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E-ISSN 2582-2160
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CrossRef DOI prefix of IJFMR is 10.36948/ijfmr

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