Formulation and Evaluation of Herbal Antimicrobial Basil Seed Gel Enriched with Tea Tree Oil

Vaishnavi N. Korane; Savita B. Lavhate; Dilksha S. Kurane; Prashant S. Kumbhar

Formulation and Evaluation of Herbal Antimicrobial Basil Seed Gel Enriched with Tea Tree Oil

Author(s)	Vaishnavi N. Korane, Savita B. Lavhate, Dilksha S. Kurane, Prashant S. Kumbhar
Country	India
Abstract	Skin infections and microbial contamination are common health concerns that require safe and effective topical treatments. The increasing use of synthetic antimicrobial agents has raised concerns regarding adverse effects and antimicrobial resistance, creating a need for natural alternatives. The present study was undertaken to formulate and evaluate a herbal antimicrobial gel enriched with basil seed gel (Ocimum basilicum) and tea tree oil (Melaleuca alternifolia) for topical application. Basil seed mucilage was extracted and utilized as a natural gelling agent, while tea tree oil was incorporated for its well-known antimicrobial properties. The gel was formulated using basil seed gel, tea tree oil, aloe vera gel, glycerin, carbopol 940, vitamin E, rose water, and suitable preservatives. The prepared formulation was evaluated for various physicochemical parameters, including appearance, pH, spreadability, viscosity, washability, phase separation, irritancy, and antimicrobial activity. The developed gel exhibited a transparent appearance, pleasant odor, smooth texture, and good consistency. The pH of the formulation was found to be 6.12, indicating compatibility with normal skin. The gel showed satisfactory spreadability, excellent washability, high viscosity, and no evidence of phase separation. Irritancy studies confirmed that the formulation was non-irritating and safe for topical use. Phytochemical screening revealed the presence of phenols, flavonoids, terpenes, and fatty acids, which contribute to its therapeutic activity. Antimicrobial evaluation demonstrated significant inhibitory activity against selected microorganisms. The results indicate that the formulated basil seed gel enriched with tea tree oil possesses desirable physicochemical characteristics, good stability, safety, and promising antimicrobial potential, suggesting its suitability as a natural topical antimicrobial formulation for skincare and wound-care applications.
Keywords	Basil Seed Gel, Tea Tree Oil, Herbal Gel, Antimicrobial Activity, Topical Formulation, Ocimum basilicum, Melaleuca alternifolia
Field	Chemistry > Pharmacy
Published In	Volume 8, Issue 4, July-August 2026
Published On	2026-07-21

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Formulation and Evaluation of Herbal Antimicrobial Basil Seed Gel Enriched with Tea Tree Oil

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