Phenotypic detection of Metallo-Beta Lactamases in Pseudomonas aeruginosa in a tertiary care hospital of Western Rajasthan

Author(s)	Mrs. Vishakha Ashopa, Dr. Prabhu Prakash
Country	India
Abstract	Introduction: Pseudomonas aeruginosais an opportunistic gram negative bacilli, often causing Hospital acquired infection. Pseudomonas spp. infection is prevalent among patients with burn wounds, cystic fibrosis, acute leukemia and intravenous drug users and urinary tract infection etc. Aim of the study:The present study was designed to determine the prevalence of Metallo -beta lactamase production in Pseudomonas aeruginosaand to evaluate the phenotypic test methods to detect the MBL production in imipenem-resistant clinical isolates of Pseudomonas aeruginosa. Materials and Methods:The present study was conducted fromSeptember 2022 to December 2024 at the department of Microbiology ,Dr.S.N Medical college,Jodhpur.It was a hospital based prospective study. Results:Totally 215,isolates of Pseudomonas aeruginosa were collected from various clinical specimens.Out of which 184 were impipenem resistant .Highest sensitivity and specificity was shown by E –test i.e 184(100%) followed by combined disc test 177 (96.17%),MHT 98(53.26%) and DDST 96(52.17%) respectively.
Keywords	Metallo-beta lactamases (MBL),Muller Hinton Agar (MHA),Combined disc test (CDT),Double disc synergy test (DDST) and E-test
Field	Medical / Pharmacy
Published In	Volume 8, Issue 4, July-August 2026
Published On	2026-08-07
DOI	https://doi.org/10.36948/ijfmr.2026.v08i04.84364

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