Development of an Efficient Protocol for In Vitro Callus Induction in Salvadora persica L.

Author(s)	Dr. Shashikant Tukaram Bandewar, Dr. Pramod Shivajirao Fuke, Dr. Tukaram Dattatray Kamble
Country	India
Abstract	Abstract: The present investigation was undertaken to establish an efficient protocol for in vitro callus induction in Salvadora persica L. Healthy leaf, nodal stem, and shoot tip explants were excised from donor plants and cultured for callus induction. The explants were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). Callus induction and proliferation varied significantly with the type of explant and the concentration of the plant growth regulator. Among the explants tested, leaf explants exhibited the highest callus induction and proliferation on MS medium supplemented with 1.0 mg L⁻¹ 2,4-D. In contrast, maximum callus formation was observed at 1.5 mg L⁻¹ and 2.5 mg L⁻¹ 2,4-D in nodal stem and shoot tip explants, respectively. The induced calli were friable and actively proliferating, and three-week-old callus cultures were subsequently used for in vitro shoot regeneration studies.
Keywords	Keywords: Salvadora persica L, callus cultures, Miswak, organogenesis, halophyte, salt tolerance.
Field	Biology > Agriculture / Botany
Published In	Volume 8, Issue 4, July-August 2026
Published On	2026-08-08

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