International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

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Volume 8 Issue 4
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Association of Dengue Virus Serotypes with Disease Severity and Clinical-Hematological Parameters: A Hospital-Based Analytical Study

Author(s) Mr. Mohammad Zulfiqar Ali, Dr. Kailash jatav
Country India
Abstract Abstract
Background: Dengue disease severity varies by viral serotype and host factors. This study examined associations between dengue virus (DENV) serotypes, disease severity, and clinical-hematological parameters.
Aims & Objectives: To investigate the molecular epidemiology of circulating dengue serotypes, identify DENV-1 to DENV-4 by RT-PCR, determine their demographic and clinical distribution, assess associations with disease severity, and generate data to strengthen dengue surveillance and public health interventions.
Methods: A hospital-based cross-sectional analytical study was conducted among 290 clinically suspected dengue patients at Index Medical College Hospital and Research Centre, Indore, from January 2023 to January 2026. Serum samples were screened using the SD BIOLINE™ Dengue Duo Rapid Test, followed by viral RNA extraction and real-time RT-PCR with the RealStar® Dengue Type RT-PCR Kit 1.0. Dengue serotypes were identified, and clinical and hematological parameters were compared according to disease severity.
Results: Real-time RT-PCR confirmed dengue infection in 67 (23.1%) patients. DENV-2 was the predominant serotype (46.3%), followed by DENV-3 (26.9%), DENV-1 (20.9%), DENV-4 (3.0%), and mixed infections (3.0%). Serotype was significantly associated with disease severity (p=0.012). Mean platelet count declined significantly from 142,800/µL in dengue without warning signs to 42,300/µL in severe dengue (p<0.001). Thrombocytopenia was the most common hematological abnormality (88.1%). DENV-2 infection, platelet count <50,000/µL, bleeding manifestations, and abdominal pain were independently associated with severe dengue.
Conclusion: DENV-2 predominated and was significantly associated with severe dengue. Molecular serotyping combined with clinical and hematological assessment may facilitate early identification of patients at risk of severe disease.
Keywords Keywords: Dengue; DENV-2; Serotyping; RT-PCR; Disease severity; Thrombocytopenia; Hematological parameters.
Field Biology > Medical / Physiology
Published In Volume 8, Issue 4, July-August 2026
Published On 2026-08-16
DOI https://doi.org/10.36948/ijfmr.2026.v08i04.85864
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