Estimation of Toxin (????-ODAP) Contents in Fresh, Stored, and Germinating Lathyrus sativus Seeds

Sarmin Sultana Shirin

doi:10.36948/ijfmr.2026.v08i05.87147

Estimation of Toxin (????-ODAP) Contents in Fresh, Stored, and Germinating Lathyrus sativus Seeds

Author(s)	Ms. Sarmin Sultana Shirin
Country	Bangladesh
Abstract	Lathyrus sativus (Grass Pea) remains an indispensable pulse crop due to its rich nutritional profile and climate adaptability; however, its dietary utility is severely limited by the neurotoxin β-N-oxalyl-α,β-diaminopropionic acid (β-ODAP). This study systematically evaluated the impact of long-term ambient storage, seed hydration (soaking), and multi-stage germination regimes on the dynamic degradation of β-ODAP and moisture retention. Fresh untreated seeds (baseline) recorded an initial moisture level of 12.20±0.30% alongside a mean toxin concentration of 0.80±0.04% dry weight. Prolonged dry storage for 12 months demonstrated negligible detoxification capacity, achieving a minor reduction of only 3.8%. In contrast, biological processing significantly enhanced toxin elimination. Imbibition via 12-hour soaking increased seed moisture to 49.50±1.10% and facilitated a 12.5% toxin loss through passive leaching. Subsequent germination further accelerated enzymatic degradation, systematically reducing β-ODAP levels to 0.50±0.03% (37.5% reduction) at 24 hours, 0.35±0.02% (56.3% reduction) at 48 hours, and reaching a minimum of 0.20±0.02% at 72 hours. Ultimately, the maximum relative neurotoxin reduction of 75.0% was accomplished during Stage III sprouting (72 hours), corresponding to a peak moisture accumulation of 72.00±1.90%. The findings establish that controlled germination is a highly sustainable, low-cost domestic protocol to effectively detoxify L. sativus seeds before dietary consumption
Keywords	Lathyrus sativus, β-ODAP, Neurolathyrism, Detoxification, Germination Kinetics, Seed Hydration, Biological Processing.
Field	Biology > Bio + Chemistry
Published In	Volume 8, Issue 5, September-October 2026
Published On	2026-09-04
DOI	https://doi.org/10.36948/ijfmr.2026.v08i05.87147

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Estimation of Toxin (????-ODAP) Contents in Fresh, Stored, and Germinating Lathyrus sativus Seeds

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