International Journal For Multidisciplinary Research

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Volume 8 Issue 5
September-October 2026

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Evaluation of the Antioxidant and Antibacterial Activities of Solvent Extracts of Glycine max (L.) Merr. Residues

Author(s) Mr. Bhushan Arun Dhande
Country India
Abstract Soybean (Glycine max (L.) Merr.) residues may serve as a potential source of naturally occurring compounds with antioxidant and antibacterial properties. The present study investigated the antioxidant activity and preliminary antibacterial potential of methanolic, ethanolic, n-hexane and distilled-water extracts of soybean residues. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay at seven concentrations ranging from 0.7 to 50 µg/mL, with ascorbic acid used as the reference antioxidant. According to the supplied graph, the n-hexane extract showed the highest estimated radical-scavenging activity at 50 µg/mL (approximately 82–83%), followed by the ethanolic extract (68–69%), methanolic extract (approximately 53%) and aqueous extract (approximately 46%). These values should be considered approximate because the original absorbance readings were unavailable for independent verification. Preliminary antibacterial screening was conducted against Staphylococcus aureus NCIM 2925 and Escherichia coli NCIM 2065 using the agar-well diffusion method, with streptomycin as the positive control. A resazurin-based microdilution assay was also reported. However, incomplete methodological details and the absence of results separated by bacterial species prevented reliable quantitative comparisons of inhibition zones and confirmation of organism-specific minimum inhibitory concentrations (MICs). Overall, the available findings indicate differences in the observed DPPH response among the solvent extracts. Further investigation using standardized experimental procedures and complete data records is necessary to validate these observations and assess the potential value of soybean residues for sustainable utilization.
Keywords Keywords: soybean residues; Glycine max; DPPH assay; antioxidant activity; antibacterial activity; resazurin assay; agricultural residue utilization
Field Biology > Agriculture / Botany
Published In Volume 8, Issue 5, September-October 2026
Published On 2026-10-13
DOI https://doi.org/10.36948/ijfmr.2026.v08i05.89652
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CrossRef DOI prefix of IJFMR is 10.36948/ijfmr

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