Antagonist Test Trichoderma Sp. Tartar Isolate Against Colletotrichum Sp. Isolates Causes of Anthracnose in Chili Peppers (Capsicum Frutescens L.)

Anastasia Naomi Enjelina Wuwung; Parluhutan Meigie Jacklien Siahaan; Dingse Lamba' Pandiangan

Antagonist Test Trichoderma Sp. Tartar Isolate Against Colletotrichum Sp. Isolates Causes of Anthracnose in Chili Peppers (Capsicum Frutescens L.)

Author(s)	MS Anastasia Naomi Enjelina Wuwung, Dr. Parluhutan Meigie Jacklien Siahaan, Prof. Dr. Dingse Lamba' Pandiangan
Country	Indonesia
Abstract	Anthracnose disease in chili peppers caused by Colletotrichum sp. is one of the diseases that can reduce the yield and quality of chili production. This study aims to test the ability of Trichoderma sp. Taratara isolate antagonists against Colletotrichum sp. in vitro, determine the viability of conidia, and observe the mechanism of antagonism that occurs. The research was conducted at the Advanced Biology Laboratory of FMIPA, Sam Ratulangi University using a laboratory experiment method with a Complete Random Design (RAL) consisting of dual culture control and treatment, five replicas each. Trichoderma sp. was explored from plantation soil in Taratara Village using the fishing method with coconuts, then purified and morphologically characterized. The viability test was carried out through observation of conidia germination after 24 hours of incubation, while the antagonist test was carried out by the dual culture method on PDA media for 7 days. The results showed that Taratara isolates had a conidia viability of 96.74 ± 3.98%. The growth of Trichoderma sp. colonies took place faster than Colletotrichum sp., with the diameter of the colony on day 7 reaching 8.5 cm and 4.7 cm, respectively. The percentage of resistance of Trichoderma sp. against Colletotrichum sp. increased during the incubation period and reached 60.43% on day 7. Observations at the interaction zone showed that the mechanisms of antagonism that were actually observed were space and nutrient competition, characterized by faster and more media-dominated growth of Trichoderma sp. and an indication of mycoparasitism. These results show that Trichoderma sp. Taratara isolate has the potential to be a biological control agent against Colletotrichum sp. causing anthracnose in chili peppers.
Keywords	Trichoderma Sp., Colletotrichum Sp., Antagonism, Dual Culture, Anthracnose
Field	Biology > Agriculture / Botany
Published In	Volume 8, Issue 5, September-October 2026
Published On	2026-10-26

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Antagonist Test Trichoderma Sp. Tartar Isolate Against Colletotrichum Sp. Isolates Causes of Anthracnose in Chili Peppers (Capsicum Frutescens L.)

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